Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 36, Issue 10, Pages 1248-1258Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2004.02.012
Keywords
liver transplantation; steatosis; alcohol; polyphenols; free radical
Funding
- NIAAA NIH HHS [AA09156] Funding Source: Medline
- NIDDK NIH HHS [P30 DK34987, K01 DK62089] Funding Source: Medline
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Fatty liver caused by ethanol decreases survival after liver transplantation in rats. This study investigated if antioxidant polyphenols from Camellia sinenesis (green tea) prevent failure of fatty grafts from ethanol-treated rats. Donor rats were given ethanol intragastrically (6 g/kg). After 20 h, livers were explanted and stored in University of Wisconsin solution for 24 h. Prior to implantation, the explanted grafts were rinsed with lactated Ringer's solution containing 0 to 60 mug/ml polyphenols. Alanine aminotransferase (ALT) release after liver transplantation was 4.5-fold higher in recipients receiving ethanol-induced fatty grafts than in those receiving normal grafts. Liver grafts from ethanol-treated donors also developed severe focal necrosis. Graft survival Was 11% in the ethanol group versus 88% for normal grafts. Polyphenol treatment at 60 mug/ml blunted ALT release by 66%, decreased necrotic areas by 84%, and increased survival to 75%. Ethanol increased alpha-(4-pyridyl-1-oxide)-N-tert. -butylnitrone free radical adducts in bile by 2.5-fold, as measured by electron spin resonance spectroscopy, and caused accumulation of 4-hydroxynonenal in liver sections, effects blunted by polyphenols. Epicatechin gallate, a major potyphenol from C. sinenesis, also decreased enzyme release, minimized pathological changes, and decreased free radical adduct formation. In conclusion, polyphenols scavenged free radicals in ethanol-induced fatty livers and decreased injury after liver transplantation. (C) 2004 Elsevier Inc. All rights reserved.
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