Journal
CARBOHYDRATE RESEARCH
Volume 339, Issue 7, Pages 1339-1346Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2004.02.025
Keywords
fucan sulfate; methylation analysis; NMR spectroscopy; ostcoclastogenesis; sea cucumber body wall
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Two types of fucan sulfate were isolated from chloroform/methanol extract of the body wall of the sea cucumber Stichopus,japonicus. One type (type A) contained 3.41 mmol fucose/g and 2.35 mmol sulfate/g, and the molecular mass was determined to be 9 kDa by gel permeation chromatography (GPC). Structural analysis suggested that type A consists of a backbone of (1 --> 3)-linked fucosyl residues that are substituted at C-4 with fucosyl residues, and that fucosyl residues are sulfated at C-2 and/or C-4. Another type (type B) contained 3.90 mmol fucose/g and 3.07 mmol sulfate/g, and the molecular mass was determined to be 32 kDa by GPC. Structural analysis showed that type B is largely composed of unbranched (1 --> 3)-linked fucosyl residues, and that sulfate substitution(s) occur at C-2 and/or C-4. The potential of both types to inhibit osteoclastogenesis was examined by an in vitro assay system, showing that both types of fucan sulfate inhibit osteoclastogenesis more than 95% at 50 mug/mL concentration. These results suggest that types A and B fucan sulfate from sea cucumber are potent inhibitors of osteoclastogenesis. (C) 2004 Elsevier Ltd. All rights reserved.
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