Journal
FEBS LETTERS
Volume 566, Issue 1-3, Pages 307-310Publisher
WILEY
DOI: 10.1016/j.febslet.2004.04.018
Keywords
polyarginine; double-stranded RNA; post-transcriptional gene silencing; RNase protection assay
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We have used cationic oligopeptide polyarginine-12mer (POA) to deliver double-stranded RNA (dsRNA), prepared in vitro, to tobacco (Nicotiana tabacum) suspension cells. POA interacts electrostatically with dsRNA to form a complex. When dsRNA for the GUS or NPTII gene was delivered into cells carrying the same genes, the corresponding mRNA was degraded. Using RNase protection assay we were able to detect 21-bp small interfering RNA in dsRNA/POA-treated cells. These results demonstrate that POA can be used to deliver dsRNA to induce post-transcriptional gene silencing in plant cells. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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