Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1036, Issue 2, Pages 95-100Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.02.088
Keywords
high-salt stacking; sweeping; on-line sample concentration; electrokinetic chromatography
Funding
- NCI NIH HHS [CA-91216] Funding Source: Medline
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High-salt stacking in electrokinetic chromatography (EKC) is defined and contrasted to the sweeping method. A recent paper argued the two methods are identical, where high concentrations of micelle in the sample were intended to mimic the effect of high-salt stacking. However, high micelle concentration in the sample matrix in EKC is analogous to using a high-conductivity sample instead of a low-conductivity sample in field amplified stacking. High-salt stacking does not require a sample free of pseuostationary phase, only a sample with a high-mobility co-ion compared to the separation buffer electrokinetic vector. High-salt stacking uses a discontinuous buffer system and should not be confused with continuous buffer stacking systems such as sweeping. (C) 2004 Elsevier B.V. All rights reserved.
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