Journal
SCIENCE
Volume 304, Issue 5674, Pages 1170-1173Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1095211
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Funding
- NIGMS NIH HHS [GM48746, R01 GM048746] Funding Source: Medline
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Bacteria use conjugation systems, a subfamily of the type IV secretion systems, to transfer DNA to recipient cells. Despite 50 years of research, the architecture and mechanism of action of the channel mediating DNA transfer across the bacterial cell envelope remains obscure. By use of a sensitive, quanti. able assay termed transfer DNA immunoprecipitation (TrIP), we identify contacts between a DNA substrate (T-DNA) and 6 of 12 components of the VirB/D4 conjugation system of the phytopathogen Agrobacterium tumefaciens. Our results de. ne the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage.
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