Journal
AQUACULTURE RESEARCH
Volume 45, Issue 9, Pages 1559-1566Publisher
WILEY
DOI: 10.1111/are.12354
Keywords
common carp; glutamine precursors; GS; expression
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Funding
- earmarked fund for China Agriculture Research System [CARS-46]
- Central-Level Non-profit Science Research Institutes Special Funds [2012NYBSJ-01]
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To evaluate if the addition of glutamine (Gln) precursors could enhance the utilization rate of plant protein on molecular level, the expressions of glutamine synthetase (GS) gene were analysed under supplementation of a-ketoglutaric acid (AKG), ornithine-a-ketoglutaric acid (OKG), glutamic acid (Glu), and Gln. The relative quantification of GS gene expression in the plant meal (PM) group was significantly lower than the fish meal (FM) group. All the four additives increased the GS gene expression. The additive that most significantly increased the GS gene expression, Gln concentration and GS activity was glutamic acid. Supplementation of AKG in plant meal can improve the GS activity and Gln concentration. Effect of OKG on the activity of GS was not significant. The GS expression was up-regulated by Gln, but had no significant effects on the Gln concentration and GS activity. In conclusion, substitution of plant protein for animal protein reduced the GS gene expression, and the supplementation of Gln precursors was capable of promoting the expression of GS gene in common carp.
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