Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 50, Issue 6, Pages 451-454Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/W04-027
Keywords
Bacillus subtilis; antifungal activity; chiA; Bacillus circulans
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Bacillus subtilis F29-3 is an antagonistic bacterium against a wide range of fungal species. In order to determine the effect of chitinase on the antifungal activity of B. subtilis F29-3, a 2.4-kb DNA fragment containing the chiA gene of Bacillus circulans WL-12 was ligated into a shuttle vector pHY300PLK and transformed into B. subtilis F29-3. A bioassay conducted on the culture supernatant showed that, in comparison to the B. subtilis control strain, B. subtilis F29-3 expressing the chiA gene exhibited a greater inhibition of spore germination of Botrytis elliptica, indicating that chitinase could enhance the antifungal function conferred by B. subtilis F29-3.
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