Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 16, Issue 3, Pages 203-213Publisher
SPRINGER
DOI: 10.1023/B:JAPH.0000048506.58928.4d
Keywords
micropropagation; polarity; buds; callus; plantlets; Gracilaria chilensis
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Regeneration studies were carried out on four morphotypes of Gracilaria chilensis found on the coast of Chile (36 - 41 degreesS). Vegetative explants were obtained from sections of apical and medial origin and were cultured in five media: filtered autoclaved seawater (FAS), Provasoli enriched seawater ( PES), PES + indole-3-acetic acid ( PIAA), PES + kinetin (PK) and PK + IAA (PKIAA). Mature female gametophyte and tetrasporophyte explants were obtained from sections of medial origin and were cultured in FAS and PES. Bud differentiation and/or callus formation were the morphogenetic responses to the wounding of the explants in all culture media. Plantlet regeneration was obtained from excised buds and calluses cultured separately.
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