Journal
AQUACULTURE RESEARCH
Volume 41, Issue 6, Pages 804-813Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2109.2009.02357.x
Keywords
Leuciscus idus L; milt; hormonal stimulation; seminal plasma; motility of spermatozoa; Ovopel
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Milt of the Leuciscus idus L. was collected from five experimental groups, and selected parameters of its quality were analysed for 36 h (group II), 60 h (group III), 84 h (group IV) and 108 h (group V), respectively, after hormonal stimulation with Ovopel (1 granule kg-1 of body weight). The control (group I) fish were not subjected to hormonal stimulation. The highest milt volume was obtained from the fish in group IV (0.70 +/- 0.55 mL), where the largest volume of milt expressed per kilogram was also obtained (3.03 +/- 1.94 mL kg-1). Significant differences were also found in milt volumes obtained between group I and groups III (P < 0.01) and IV (P < 0.05). The highest percentage of motile spermatozoa was found in the milt of group IV (59%); significant differences were found between group I and groups II (P < 0.01) and III (P < 0.001). The value of osmotic pressure of seminal plasma was the highest in group IV (203.19 +/- 37.63 mOsm kg-1), and the lowest in group I (118.31 +/- 41.13 mOsm kg-1). Parameters determining milt quality and quantity indicate that the period of 60-84 h after hormonal stimulation with Ovopel is optimal for obtaining milt from ide.
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