Journal
AQUACULTURE RESEARCH
Volume 41, Issue 1, Pages 27-34Publisher
WILEY
DOI: 10.1111/j.1365-2109.2009.02298.x
Keywords
Vibrio alginolyticus; multiplex PCR; collagenase gene; ompK gene; toxR gene
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Funding
- Bureau of Science and Technology of Guangdong Province of the China
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The bacterial strains obtained from various origins were tested with the novel primers targeting the collagenase gene, ompK gene and toxR gene to establish a multiplex polymerase chain reaction (PCR) method. These primers successfully recognized all virulent strains of Vibrio alginolyticus, but the avirulent strains were not recognized by the multiplex PCR because of lack of the collagenase and toxR genes. In a 50 mu L multiplex PCR mixture, the lowest detection limit is 8.8 x 102 cells of virulent strains of V. alginolyticus. The multiplex PCR method was successfully developed to identify virulent strains of V. alginolyticus, and provides a rapid, sensitive, specific and reliable technology for diagnosing virulent strains of V. alginolyticus. Therefore, the novel multiplex PCR in the present paper can be useful for any laboratory working with vibriosis detection of aquatic animals.
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