Journal
AQUACULTURE RESEARCH
Volume 41, Issue 10, Pages 1452-1461Publisher
WILEY
DOI: 10.1111/j.1365-2109.2009.02436.x
Keywords
antivibrio compound; N-methyl-1-hydroxyphenazine; liquid chromatography-mass spectroscopy; nuclear magnetic resonance spectroscopy; haemocyte culture; aquaculture
Categories
Funding
- Department of Ocean Development, Ministry of Science and Technology [DOD/11/MRDF/4/3/UNI/97(p-8)]
- Department of Biotechnology, Ministry of Science and Technology [BT/PR4012/AAQ/03/204/2003]
- Government of India, NewDelhi
- DOD
- DBT
Ask authors/readers for more resources
Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at different temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, purified and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UV-Vis and nuclear magnetic resonance spectroscopy and identified as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC50 value was found to be 1.4 +/- 0.31 mg L-1. The compound was found to be bacteriostatic at 0.5 mg L-1. Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available