Journal
BIOCHEMICAL JOURNAL
Volume 380, Issue -, Pages 353-360Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20040062
Keywords
cytochrome P450; cytochrome P450 2D6 (CYP2D6); dextromethorphan; drug metabolism; ligand-binding property; mutagenesis
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Although the residues that determine the preference of CYP2D6 (cytochrome P450 2D6) for compounds containing a basic nitrogen are well characterized, the contribution of other active site residues to substrate binding and orientation is less well understood. Our structural model of CYP2D6 identifies the aromatic residue Phe(120) as a likely major feature of the active site. To examine the role of Phe(120), mutants of CYP2D6 in which this residue has been substituted by alanine, leucine, tyrosine, serine, histidine, tryptophan or methionine residues have been prepared in bacterial membranes co-expressing human cytochrome NADPH cytochrome P450 oxidoreductase. The mutants have been characterized using the prototypical bufuralol F hydroxylase and dextromethorphan O- and N-demethylase activities of CYP2D6. Larger effects on K. values are observed for dextromethorphan O-demethylation than for butfuralol I'hydroxylation, indicating that the Phe(120) side chain is more important in dextromethorphan than in bufuralol binding. A role for this side chain in determining the regiospecificity of substrate oxidation was indicated by changes in the relative rates of O- and N-demethylation of dextromethorphan and, notably, by the formation of 7-hydroxy dextromethrophan, a novel dextromethorphan metabolite, in mutants in which it had been substituted. Computational studies of dextromethorphan binding to the active site of the Phe(120) --> Ala mutant were carried out to throw light on the way in which the removal of this side chain leads to different modes of ligand binding.
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