Journal
BIOTECHNOLOGY LETTERS
Volume 26, Issue 11, Pages 933-937Publisher
SPRINGER
DOI: 10.1023/B:bile.0000025906.95694.1a
Keywords
cytoplasmic enzyme; electro extraction; electropulsation; flow process; Schizosaccharomyces pombe
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Flow treatment of the yeast, Schizosaccharomyces pombe, with high intensity electric field pulses released intracellular enzymes such as glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase. Over 70% of the total activity was liberated within 4 h after pulse application. The optimal field intensities were considerably higher than that needed for irreversible plasma membrane permeabilization.
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