Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 22, Issue 2, Pages 173-177Publisher
SPRINGER
DOI: 10.1007/BF02772724
Keywords
DNA isolation; polyphenols; polysaccharides; Vitis vinifera
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We have developed a simple and highly efficient protocol for isolating large quantities (150-400 mug/g leaf tissue) of high-quality DNA from fresh and frozen Vitis vinifera leaves. Isolated DNA is essentially free of polysaccharides, polyphenols, and other major contaminants as judged by viscosity, clear colors A(260/280) ratio, digestibility by restriction enzymes for Southern blot analysis, and PCR suitability.
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