Journal
BIOTECHNOLOGY LETTERS
Volume 26, Issue 11, Pages 891-895Publisher
SPRINGER
DOI: 10.1023/B:bile.0000025898.15309.0e
Keywords
duplex scorpion primer; real-time quantitative PCR; telomerase activity; TRAP assay
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A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase activity could be obtained (R-2 = 0.994). The requirement of post-PCR steps is thus obviated.
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