Journal
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
Volume 21, Issue 6, Pages 1210-1215Publisher
OPTICAL SOC AMER
DOI: 10.1364/JOSAB.21.001210
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We demonstrate a new method of determining the three-dimensional dipole orientations of single molecules by direct imaging of the emission patterns in the back focal plane of a high-numerical-aperture objective lens. We compare the reconstructed emission-dipole orientations with a previously established method of absorption-dipole mapping. We find that, for a given number of emitted photons, emission pattern imaging provides better accuracy (1degrees-2degrees) than absorption-dipole mapping of single molecules. Compared with some other methods for emission-dipole mapping, the presented method is (1) less sensitive to optical aberrations and adjustment and (2) data analysis is simplified because radiation patterns can be expressed in a simple analytical form. (C) 2004 Optical Society of America.
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