Journal
AQUACULTURE
Volume 356, Issue -, Pages 169-175Publisher
ELSEVIER
DOI: 10.1016/j.aquaculture.2012.05.019
Keywords
Carp; mRNA expression; Quantitative PCR; Cytokine; Yeast extract
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (Monbukagakusho), Japan
- JSPS Asian CORE Program and Scientific Research [23580257]
- Grants-in-Aid for Scientific Research [12J10303, 23580257] Funding Source: KAKEN
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Cytokines are important regulators of the immune system and investigation of their functions may prove useful for the development of vaccines and immunostimulants for aquaculture. We therefore investigated the cytokine [interleukin (IL)-1 beta, IL-10, IL-12 p35 and p40, tumor necrosis factor (TNF)-alpha, CXC-chemokine and interferon (IFN)-alpha and gamma] responses of the common carp, Cyprinus carpio L., upon treatment with a commercial baker's yeast extract (CW-I) that contained nucleotides and beta-glucan. Additionally, to confirm that the CW-I-induced cytokines were functional, we also assessed the effect of CW-I administration on superoxide anion production and phagocytic activities of head kidney leucocytes and resistance to Aeromonas hydrophila infection in the common carp. Our results demonstrate that baker's yeast extract-treated fish displayed a significant up-regulation of pro-inflammatory cytokine (IL-1 beta, IL-12 p35 and p40, TNF-alpha, CXC-chemokine, IFN-gamma 2) gene expression and a down-regulation of anti-inflammatory cytokine (IL-10) gene expression. Furthermore, significantly increased phagocytic activity and superoxide anion production in kidney cells, and resistance to a bacterial pathogen, were observed in the yeast extract-treated fish compared to non-treated fish. The current study indicates the immunostimulatory effects of a baker's yeast extract rich in nucleotides and beta-glucan on the carp immune system and supports its potential use in aquaculture. (C) 2012 Elsevier B.V. All rights reserved.
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