Journal
VIRUS RESEARCH
Volume 102, Issue 1, Pages 27-35Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2004.01.012
Keywords
respiratory syncytial virus; vesicular stomatitis virus; parainfluenza virus; RNA interference; siRNA; host-virus interaction
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Funding
- NEI NIH HHS [EY013826] Funding Source: Medline
- NIAID NIH HHS [AI049682] Funding Source: Medline
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Our laboratory provided the first proof-of-concept that double-stranded short interfering RNA (ds-siRNA) can act as potent and specific antiviral agents. Designed against specific mRNAs of nonsegmented negative-stranded RNA (NNR) viruses, siRNAs abrogated expression of the corresponding viral proteins, and generated the predicted viral phenotypes. Knockdown was demonstrated across different genera: respiratory syncytial virus (RSV), a pneumovirus;, vesicular stomatitis virus (VSV), a rhabdovirus; and human parainfluenza virus (HPIV), a paramyxovirus. The targeted genes could have a wide range of functions, thus documenting the versatility of the technique. Interestingly, antisense single-stranded siRNA (ss-siRNA) was also effective, albeit at a higher concentration. NNR viral genomic and antigenomic RNA, which are encapsidated by nucleocapsid protein and serve as templates for viral RNA-dependent RNA polymerase, were resistant to siRNA. Together, siRNAs offer complementary advantages over traditional mutational analyses that are difficult to perform in NNR viruses, and are also an important new tool to dissect host-virus interactive pathways. (C) 2004 Elsevier B.V. All rights reserved.
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