Journal
AQUACULTURE
Volume 319, Issue 3-4, Pages 311-314Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2011.04.003
Keywords
WSSV; siRNA; E. coli; Marsupenaeus japonicus
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Funding
- National Natural Science Foundation of China [30830084, 31001247]
- Hi-Tech Research and Development Program of China (863 program of China) [2010AA09Z403]
- Ministry of Agriculture, China [201103034]
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White spot syndrome virus (WSSV) has been a major viral pathogen responsible for large economic losses to shrimp aquaculture all over the world. The RNAi mediated by siRNA contributes a novel strategy to control this viral disease. However, the efficient approach to deliver the siRNA into shrimp in vivo remains to be addressed. In our study, the vp28-siRNA, having a specific activity against WSSV, was expressed in an RNase III-deficient strain of Escherichia coli HT115 (DE3). Then the inactivated bacteria expressing the vp28-siRNA were delivered into Marsupenaeus japonicus shrimp by injection. The results showed that the vp28-siRNA could be detected in shrimp hemocytes. As shown by quantitative PCR, the vp28-siRNA inhibited the replication of WSSV in shrimp, which presented a better antiviral activity of siRNA. Further evidence indicated that the mortality of WSSV-infected shrimp was significantly reduced (P < 0.01) by the vp28-siRNA. Therefore this study presented a novel efficient therapeutic approach to control WSSV by using siRNA expressed in bacteria. (C) 2011 Published by Elsevier B.V.
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