Journal
JOURNAL OF BACTERIOLOGY
Volume 186, Issue 11, Pages 3392-3398Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.186.11.3392-3398.2004
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Funding
- NIGMS NIH HHS [GM36718, R01 GM036718] Funding Source: Medline
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The Bacillus subtilis rocG gene, encoding catabolic glutamate dehydrogenase, was found to be subject to direct CcpA-dependent glucose repression. The effect of CcpA required the presence of both the HPr and Crh proteins. The primary CcpA binding site was identified by mutational analysis and DNase I footprinting. In the absence of inducers of the Roe pathway, rocG was still expressed at a low level due to readthrough transcription. CcpA-dependent repression of rocG readthrough transcription proved to contribute to the slow growth rate of B. subtilis cells in glucose-glutamate medium. Increased readthrough expression of rocG was shown to be partially responsible for the growth defect of ccpA strains in glucose-ammonium medium.
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