Anaesthesia of Atlantic cod (Gadus morhua) - Effect of pre-anaesthetic sedation, and importance of body weight, temperature and stress

The efficacy of the anaesthetic agents benzocaine, metacaine (MS-222). metomidate and 2-phenoxyethanol was studied in Atlantic cod (Gadus morhua) with average body weights of 10 4 g. 99 33 g and 1022 274 g at water temperatures of 8 degrees C and 16 degrees C. The agents were tested individually and as combination anaesthesia comprising pre-anaesthetic sedation with a low dosage of metomidate or 2-phenoxyethanol followed by anaesthesia with benzocaine or MS-222. All agents were administered through bath immersion with an exposure time of 5 min. The different treatments resulted in average induction and recovery times ranging from 52 +/- 6 s to 182 +/- 16 s and 77 +/- 26 s to 659 +/- 46 s respectively. Induction and recovery times varied in relation to water temperature and were generally shorter at 16 degrees C for all weight groups and treatments compared to 8 degrees C. For benzocaine and MS-222 induction and recovery times were found to increase with increasing body weight. For metomidate the recovery time increased with increasing weight whereas there were no weight related differences in induction time. No differences in either induction or recovery times associated to body weight were found for 2-phenoxyethanol. Acute stress prior to anaesthesia with MS-222 resulted in significantly shorter induction time and prolonged recovery time, as well as deeper anaesthetised fish. The dosage of MS-222 had to be reduced in order to avoid mortality in fish subjected to acute stress. Combination anaesthesia allowed a reduction of the dosages used for inducing anaesthesia and produced markedly reduced recovery times compared to agents administered individually. (C) 2009 Elsevier B.V. All rights reserved.