Effect of light on oxygen consumption and ammonia excretion in Haliotis discus discus, H. gigantea, H. madaka and their hybrids

Oxygen consumption and ammonia excretion rates of three abalone species, Haliotis discus discus, H. gigantea, H. madaka and their hybrids were measured at 20 degrees C by incubating for 3 h under dark and light (N=9-13 for each species and hybrid). Animals were fasted before and during the experiment and measurements were made first under dark followed by light on the next day. The rates increased with the increase in body weight and were higher under light than dark. On average, H. discus discus had higher oxygen consumption (DD; dark=0.039, light=0.04 ml/g/h) than H. gigantea (G; D=0.033, L=0.036) and H. madaka (M; D=0.034, L=0.035); the hybrids had varied patterns with respect to their parental species [DDxM (D=0.032, L=0.038); MxDD (D=0.03, L=0.038); GxDD (D=0.035, L=0.04) and DDxG (D=0.03, L=0.034), mother first]. M (0.261, 0.298 mu mol/g/h) had the highest ammonia excretion rate while G (0.162; 0.264) and DD (0.229; 0.232) had the lowest under dark and light, respectively. The hybrids had varied patterns in comparison with their parents (DD x M = 0.247, 0.32;M x DD=0.177, 0.28; DD x G = 0.249, 0.364 and G x DD = 0.116, 0.155). The O/N ratios under both conditions in all species and hybrids indicated that they had carbohydrate dominated metabolism. Results demonstrated physiological variability among the species and hybrids indicating necessity of different strategies for their management and aquaculture. (C) 2008 Published by Elsevier B.V.