Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 98, Issue 6, Pages 991-994Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2004.02.014
Keywords
chromium(III) complex; BSA; tryptophan absorption; forster type fluorescence energy transfer
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A chromium(III) complex, transdiaqua [N,N'-propylenebis(salicylideneimino)chromium(III)]perchlorate ([Cr(salprn)(H2O)(2)] ClO4) in the presence of sodium azide and upon photoexcitation was found to bring about non-selective cleavage of bovine serum albumin (BSA). Electron paramagnetic resonance (EPR) evidence has been obtained for the formation of a Cr(V) species upon photolysis of a solution containing the chromium(III) complex and sodium azide. This Cr(V) species non-selectively cleaves BSA. The fluorescence excitation spectrum of BSA-[Cr(salprn)(H2O2](+) adduct showed a band at lambda(ex)(max) = 370 nm due to charge transfer transition of the chromium(III) complex as well as a prominent band at 290 nm attributable to tryptophan absorption. This indicated an efficient Forster type fluorescence energy transfer (FRET) from the tryptophan residues to the chromium(III) complex indicating that the Cr(III) complex binds in the vicinity of the tryptophan residue. (C) 2004 Elsevier Inc. All rights reserved.
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