Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 70, Issue 6, Pages 3776-3780Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.6.3776-3780.2004
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Giardia cysts in 131 raw wastewater samples from Milwaukee, Wis., were genotyped by sequence analysis of the triosephosphate isomerase gene which showed the presence of two distinct genotypes (assemblages A and B) of Giardia duodenalis. Of the 131 samples, 111 belonged to assemblage A, and the remaining samples belonged to assemblage B. A high degree of genetic polymorphism was evident within the assemblage B cluster, with 10 distinct subgenotypes identified, eight of which have not been reported before.
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