Journal
ARCHIVES OF MICROBIOLOGY
Volume 181, Issue 6, Pages 391-397Publisher
SPRINGER
DOI: 10.1007/s00203-004-0668-2
Keywords
2,6-dihydroxybenzoate decarboxylase; 2,6-dihydroxybenzoate; decarboxylation; carboxylation; Agrobacterium tumefaciens IAM12048
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A nonoxidative decarboxylase, 2,6-dihydroxybenzoate decarboxylase, was found in Agrobacterium tumefaciens IAM12048. The enzyme activity was induced specifically by 2,6-dihydroxybenzoate. The purified enzyme was a homotetramer of identical 38 kDa subunits. The purified decarboxylase catalyzed the nonoxidative decarboxylation of 2,6-dihydroxybenzoate and 2,3-dihydroxybenzoate without requiring any cofactors. In the presence of KHCO3, the enzyme also catalyzed the regioselective carboxylation of 1,3-dihydroxybenzene into 2,6-dihydroxybenzoate at a molar conversion ratio of 30%.
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