Journal
ANALYTICAL BIOCHEMISTRY
Volume 329, Issue 1, Pages 85-90Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2004.01.032
Keywords
glucose oxidase; layer-by-layer; electron transfer; enzymatic activity; biosensor
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Glucose oxidase was embedded in organic films through a layer-by-layer approach, where the enzyme demonstrated significantly enhanced electron-transfer reactivity and finely tuned enzymatic activity. An unmediated, reagentless glucose biosensor was accordingly prepared with two polyethylenimine/glucose oxidase bilayers-modified pyrolytic graphite electrode. A calibration linear range of glucose was 0.5-8.9 mM with a detection limit of 50 muM and sensitivity of 0.76 muA mM(-1). (C) 2004 Elsevier Inc. All rights reserved.
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