Journal
MOLECULAR CELL
Volume 14, Issue 5, Pages 667-673Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2004.05.013
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Previous work demonstrated the removal of nucleosomes from the PHO5 promoter upon transcriptional activation in yeast. Removal could occur by nucleosome disassembly or by sliding of nucleosomes away from the promoter. We have now activated the PHO5 promoter on chromatin circles following excision from the chromosomal locus. Whereas sliding would conserve the number of nucleosomes on the circle, we found that the number was diminished, demonstrating chromatin remodeling by nucleosome disassembly.
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