Spectroelectrochemical determination of the redox potential of p700 in spinach with an optically transparent thin-layer electrode

Optimization of experimental conditions for spectroelectro-chemistry with an optically transparent thin-layer electrode allowed us to determine the redox potential of spinach P700, the primary electron donor of photosystem I, to be +469 mV vs. SHE with significantly high reproducibility (+/-2 mV for 12 independent samples).