Synergetic inhibition of genistein and D-glucose on α-glucosidase

Synergetic inhibitory effect of genistein (I-2) and D-glucose (I-1) on alpha-glucosidase has been studied with kinetics method. it was concluded that the inhibitory effect was much greater when I-2 and I-1 were both added to the reactant solution simultaneously than that they were added, respectively, which suggesting the inhibitors bind to the different sites of alpha-glucosidase at the same time, and demonstrating synergetic inhibition. (C) 2004 Published by Elsevier Ltd.