Kinetic spectrophotometric method for simultaneous determination of selenium and tellurium using partial least squares calibration

A kinetic-spectrophotometric method for the simultaneous determination of Se(IV) and Te(IV) is described. The method is based upon the catalytic effect of these cations on the reaction of toluidine blue with sulfide. Partial least squares calibration method was employed for the data manipulation and analysis. The concentrations were varied between 0.02-0.24 and 0.01-0.08 mug/ml for Se(IV) and Te(W), respectively. Cross-validation method was used to select the optimum number of factors (NF). The root mean square errors of difference for selenium and tellurium were 1.2 and 1.7 mug/ml, respectively. Application of the method to artificial samples and several mixtures of standard solutions of Se(IV) and Te(IV) were performed and satisfactory results were obtained. (C) 2004 Elsevier B.V. All rights reserved.