Characterization and determination of stability constants of copper(II)-L-histidine complexation system by using multivariate curve resolution method of visible spectra and two hard modeling methods in aqueous solutions

Multivariate curve resolution, alternating least-squares is applied to spectra data obtained in the study of Cu(II) complexation by L-histidine. The combination of several chemometric techniques based on factor analysis (FA), singular value decomposition (SVD), evolving factor analysis (EFA), and multivariate curve resolution with constrained alternating least-squares (ALS) is used to determine the number of species and their distribution diagram. This multivariate analysis data treatment simultaneously reveals the species Cu, CuL, CuLH, CuL2, CuL2H, and CuLOH, through the calculated concentration profiles and allows the assignment of numerically obtained pure individual spectra. Formation constants of these species were calculated by hard-modeling methods applied potentiometric and spectrophotometric measurements. (C) 2004 Elsevier B.V. All rights reserved.