Journal
JOURNAL OF IMMUNOLOGY
Volume 172, Issue 12, Pages 7425-7431Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.172.12.7425
Keywords
-
Categories
Funding
- NCRR NIH HHS [RR 00164] Funding Source: Medline
- NHLBI NIH HHS [HL 61934] Funding Source: Medline
- NIDA NIH HHS [DA 016029] Funding Source: Medline
Ask authors/readers for more resources
Dendritic cells (DC) play a critical role in adaptive immunity by presenting Ag, thereby priming naive T cells. Specific DC-binding peptides were identified using a phage display peptide library. DC-peptides were fused to hepatitis C virus nonstructural protein 3 (NS3) while preserving DC targeting selectivity and Ag immunogenicity. The NS3-DC-peptide fusion protein was efficiently presented to CD4(+) and CD8(+) T cells derived from hepatitis C virus-positive blood cells, inducing their activation and proliferation. This immunogenic fusion protein was significantly more potent than NS3 control fusion protein or NS3 alone. In chimeric NOD-SCID mice transplanted with human cells, DC-targeted NS3 primed naive CD4(+) and CD8(+) T cells for potent NS3-specific proliferation and cytokine secretion. The capacity of peptides to specifically target immunogenic Ags to DC may establish a novel strategy for vaccine development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available