Journal
BIOCHEMICAL JOURNAL
Volume 380, Issue -, Pages 875-879Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20040096
Keywords
Ca2+ sensor; endocrine cell exocytosis; doublestranded RNA-mediated interference (RNAi); PC12 cell; synaptotagmin
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Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Syt isoforms acts as the major Ca2+ sensor for dense-core vesicle exocytosis. In the present study, it has been shown by immunoaffinity purification and immunocytochemistry that Syts I and IX, but not Syt IV, are present on the same secretory vesicles in PC12 cells. Silencing of Syt IX with specific small interfering RNA significantly reduced high KCl-dependent neuropeptide Y secretion from PC12 cells, whereas silencing of SytI with specific small interfering RNA had no significant effect. The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt 1, is indispensable for the regulation of Ca2+-dependent dense-core vesicle exocytosis in PC12 cells.
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