Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 101, Issue 1-2, Pages 224-230Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2004.03.002
Keywords
enzyme electrode; glucose oxidase poly(m-phenylenediamine); pulse galvanostatic method
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A new technique termed the pulse galvanostatic method (PGM) is described for constructing a glucose sensor by electropolymerization of m-phenylenediamine in the phosphate buffer solution containing in-phenylenediamine and glucose oxidase (GO(x)). The conditions for fabrication of the poly(m-phenylenediamine) glucose oxidase (PMPD GO(x)) electrode were optimized as follows: t(on)/t(off), 1; frequency, 10; mean current density, 1 mA cm(-2); concentration of the GO(x), 5 g l(-1). The morphology of PMPD GO(x) electrode prepared by PGM under the optimized conditions shows porous structure. The PMPD GO(x) electrode has short response time, high response current, good selectivity and stability. The maximum response current density and Michaelis-Menten constant of the PMPD GO(x) electrode, based on the calculation of the kinetic parameters, are 231.4 muA cm(-2) and 15.33 mM, respectively. (C) 2004 Elsevier B.V. All rights reserved.
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