Journal
MACROMOLECULAR BIOSCIENCE
Volume 4, Issue 6, Pages 610-615Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200400008
Keywords
biodegradable; chitin; enzymes; graft copolymers; lectins
Ask authors/readers for more resources
The molecular-recognition abilities of a water-soluble chitin derivative, chitin-graft-polysarcosine (2) were investigated using chitinase, lysozyme, and wheat germ agglutinin (WGA). The enzymatic degradabilities of 2 were evaluated using chitinase and lysozyme. The molecular weight of those compounds of 2 with a higher affinity toward water decreased rapidly, as compared with partially deactylated chitin (1). The H-1 NMR spectrum of the low-molecular-weight fraction, yielded after lysozymic hydrolysis, indicated that saccharide residues in the chitinous backbone were specifically recognized by the lysozyme, then beta-glycosidic linkages in the backbone of graft copolymer 2 toward the lectin WGA was elucidated by the enyzme-linked lectin-binding assay (ELLA). It was revealed that the graft copolymer with a lower degree of substitution (DS) value efficiently interacted with WGA. Interestingly, a graft copolymer having longer poly-sarcosine side chains showed higher recognition ability toward WGA than that having short side chains.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available