Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 340, Issue 1, Pages 97-114Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2004.04.039
Keywords
assembly; chemical cross-linking; intermediate filament; analytical ultracentrifugation; vimentin
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We have developed an assembly protocol for the intermediate filament (IF) protein vimentin based on a phosphate buffer system, which enables the dynamic formation of authentic IFs. The advantage of this physiological buffer is that analysis of the subunit interactions by chemical crosslinking of internal lysine residues becomes feasible. By this system, we have analyzed the potential interactions of the coiled-coil rod domains with one another, which are assumed to make a crucial contribution to IF formation and stability. We show that headless vimentin, which dimerizes under low salt conditions, associates into tetramers of the A(22)-type configuration under assembly conditions, indicating that one of the effects of increasing the ionic strength is to favor coil 2-coil 2 interactions. Furthermore, in order to obtain insight into the molecular interactions that occur during the first phase of assembly of full-length vimentin, we employed a temperature-sensitive variant of human vimentin, which is arrested at the unit-length filament (ULF) state at room temperature, but starts to elongate upon raising the temperature to 37 degreesC. Most importantly, we demonstrate by cross-linking analysis that ULF formation of A(22) and A(12) cross-linking products in mature IFs, however, indicates that major rearrangements do occur during the longitudinal annealing and radial compaction steps of IF assembly. (C) 2004 Elsevier Ltd. All rights reserved.
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