Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1040, Issue 2, Pages 169-178Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2004.04.002
Keywords
Rhizoma chuanxiong; liquid chromatography; comprehensive two-dimensional; Chinese medicines
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A comprehensive two-dimensional liquid chromatographic separation system based on the combination of a CN column and an ODS column is developed for the separation of components in a traditional Chinese medicine (TCM) Rhizoma chuanxiong. Two columns are coupled by a two-position, eight-port valve equipped with two storage loops and controlled by a computer. The effluent is detected by both the diode array detector and atmospheric pressure chemical ionization (APCI) mass spectrometer. More than 52 components in the methanol extract of R. chuanxiong were resolved and 11 of them were preliminary identified according to their UV and mass spectra. (C) 2004 Elsevier B.V. All rights reserved.
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