4.4 Article

4Pi-microscopy of the Golgi apparatus in live mammalian cells

Journal

JOURNAL OF STRUCTURAL BIOLOGY
Volume 147, Issue 1, Pages 70-76

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2003.10.006

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We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at similar to100nm resolution. (C) 2003 Elsevier Inc. All rights reserved.

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