Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 81, Issue 7, Pages 647-651Publisher
AMER OIL CHEMISTS SOC A O C S PRESS
DOI: 10.1007/s11746-004-956-x
Keywords
Aleurites fordii; linolenic acid; omega-3 fatty acid desaturase; Saccharomyces cerevisiae; tung oil
Categories
Ask authors/readers for more resources
Tung oil is an industrial drying oil containing ca. 90% PUFA. We previously reported on enzymes required for the synthesis of linoleic (6% of FA) and eleostearic (80%) acids and here describe the cloning and functional analysis of an omega-3 FA desaturase (FAD3) required for the synthesis of linolenic acid (1%). The lung FAD3 cDNA was identified by screening a lung seed cDNA library using the polymerase chain reaction and degenerate primers encoding conserved regions of the FAD3 enzyme family. Expression of this cDNA in yeast cells, cultured in the presence of linoleic acid, resulted in the synthesis and accumulation of linolenic acid, which accounted for up to 18% w/w of total cellular FA. Tung FAD3 activity was significantly affected by cultivation temperature, with the greatest amount of linolenic acid accumulating in yeast cells grown at 15degreesC. The amount of linolenic acid synthesized in yeast cells by lung FAD3 is ca. 10-fold higher than that observed by expression of a rapeseed (Brassica napus) FAD3 in yeast, suggesting that lung FAD3 might be useful for biotechnological production of omega-3 FA in transgenic organisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available