Journal
GENES & DEVELOPMENT
Volume 18, Issue 13, Pages 1618-1629Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1200804
Keywords
G-quartet; G-tetraplex; immunoglobulin switch region; telomere/transcription bubble
Categories
Funding
- NICHD NIH HHS [T32 HD07149, T32 HD007149] Funding Source: Medline
- NIGMS NIH HHS [R01 GM031693, R01 GM39799, R01 GM065988, R01 GM039799, R56 GM031693, R01 GM65988, GM31693] Funding Source: Medline
Ask authors/readers for more resources
We show that intracellular transcription of G-rich regions produces novel DNA structures, visible by electron microscopy as large (150-500 bp) loops. These G-loops are formed cotranscriptionally, and they contain G4 DNA on one strand and a stable RNA/DNA hybrid on the other. G-loop formation requires a G-rich nontemplate strand and reflects the unusual stability of the rG/dC base pair. G-loops and G4 DNA form efficiently within plasmid genomes transcribed in vitro or in Escherichia coli. These results establish that G4 DNA can form in vivo, a finding with implications for stability and maintenance of all G-rich genomic regions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available