Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 290, Issue 1-2, Pages 69-80Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2004.04.009
Keywords
yeast display; scFv library; flow cytometry
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Yeast display of antibody fragments has proven to be an efficient and productive means for directed evolution of single-chain Fv (scFv) antibodies for increased affinity and thermal stability and, more recently, for the display and screening of a nonimmune scFv and immune Fab libraries. A major strength of yeast display as a novel antibody discovery platform is the ability to characterize the binding properties, i.e., the affinity and epitope binding characteristics, of a clone without the need for subcloning, expression and purification of the scFv. This review focuses on novel attributes of yeast display for antibody engineering endeavors. (C) 2004 Elsevier B.V. All rights reserved.
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