Journal
CLINICAL AND EXPERIMENTAL ALLERGY
Volume 34, Issue 7, Pages 1131-1136Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.1365-2222.2004.02004.x
Keywords
basement membrane zone; collagen I; house dust mite allergen (Dermatophagoides farinae); ozone; perlecan; recovery; remodelling
Categories
Funding
- NCRR NIH HHS [RR000169] Funding Source: Medline
- NIEHS NIH HHS [ES05707, ES06700, P01 ES00628, P01 ES11617, ES04311] Funding Source: Medline
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Background In previous studies, we showed that repeated exposure to (1) house dust mite allergen (HDMA) (Dermatophagoides farinae) caused thickening of the basement membrane zone (BMZ) and (2) HDMA+ozone (O-3) caused depletion of BMZ perlecan and atypical development of BMZ collagen (irregular thin areas < 2.0 μm in width). Objective The purpose of this study was to determine if these remodelling changes were reversible after 6 months of recovery. Methods Rhesus monkeys were exposed to a regimen of HDMA and or O-3 or filtered air (FA) for 6 months. After the exposure protocol was completed FA and O-3 groups were allowed to recover in FA for 6 months. The HDMA and HDMA+O-3 exposure groups recovered in a modified environment. They were re-exposed to HDMA aerosol for 2 h at monthly intervals during recovery in order to maintain sensitization for pulmonary function testing. To detect structural changes in the BMZ, collagen I and perlecan immunoreactivity were measured and compared to data from the previous papers. Results The remodelled HDMA group had a significantly thicker BMZ and after 6 months of recovery the width had not regressed. In the remodelled BMZ of the HDMA+O-3 group, perlecan had returned to the BMZ after 6 months of the recovery protocol, and the thin, irregular, collagen BMZ had been resolved. Conclusion In summary, this study has shown that: (1) The width of the remodelled HDMA BMZ did not regress during a recovery protocol that included a sensitizing dose of HDMA. (2) The atypical collagen BMZ in the HDMA+O-3 BMZ was resolved in the absence of O-3. (3) Depletion of perlecan from the BMZ by O-3 was reversed by recovery in the absence Of O-3.
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