Journal
JOURNAL OF PROTEOME RESEARCH
Volume 3, Issue 4, Pages 841-850Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr049937f
Keywords
membrane proteins; transmembrane; MALDI MS/MS; lectin affinity chromatography; Con A; WGA; glycoproteins
Categories
Ask authors/readers for more resources
The aim was to determine the proportion of membrane glycoproteins captured using concanavalin A or wheat germ agglutinin lectin affinity chromatography. Digests of the isolated proteins were separated by reversed-phase liquid chromatography and analyzed by matrix-assisted laser desorption tandem mass spectrometry. The two lectins identified different groups of proteins with a broad range of molecular mass and p/ values, including a number of proteins that overlapped the two groups. Approximately 30% of the proteins were positively identified as containing domains that were predicted using standard bioinformatics methods to be characteristic of integral membrane proteins. This approach represents an effective method of surveying the membrane protein pool of mammalian cells for subsequent proteomic analysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available