Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 29, Issue 3, Pages 339-349Publisher
SPRINGER
DOI: 10.1023/B:JNMR.0000032611.72827.de
Keywords
dipolar coupling; disulfide bond; lanthanide ion; molecular alignment; NMR
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A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual N-15, H-1-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag.
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