Journal
JOURNAL OF VIROLOGY
Volume 78, Issue 13, Pages 7284-7287Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.78.13.7284-7287.2004
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Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct central memory CD62L(+) phenotype.
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