Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 11, Issue -, Pages 314-318Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0909049504014086
Keywords
SAXS; liquid chromatography; Guinier; aggregation
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Funding
- NCRR NIH HHS [RR-08630] Funding Source: Medline
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Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.
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