Journal
JOURNAL OF LIPID RESEARCH
Volume 45, Issue 7, Pages 1355-1363Publisher
ELSEVIER
DOI: 10.1194/jlr.D300040-JLR200
Keywords
reversed-phase liquid chromatography/electrospray ionization/mass spectrometry; negative ionization; counter-ion; sn-1 and sn-2 fatty acid positions; Corynebacterium species
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This work set out to optimize the detection and separation of several phospholipid molecular species on a reversed-phase column with the use of an electrospray ionization/mass spectrometry-compatible counter-ion. An application of this technique concerned a qualitative and quantitative analysis of bacterial membrane phospholipids extracted from Corynebacterium species strain 8. The phospholipid classes of strain 8 were identified as phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, and a peculiar lipid compound, acyl phosphatidylglycerol. Most of the molecular species structures were elucidated, and regarding phosphatidylglycerol, the fatty acid positions were clearly determined with the calculation of the sn-2/sn-1 intensity ratio of the fatty acyl chain fragments.-Mazzella, N., J. Molinet, A. D. Syakti, A. Dodi, P. Doumenq, J Artaud, and,I-C. Bertrand. Bacterial phospholipid molecular species analysis by ion-pair reversed-phase HPLC/ESI/MS.
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