Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 147, Issue 1, Pages 3-11Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1047-8477(03)00125-4
Keywords
third harmonic generation; multiphoton microscopy; polarization microscopy
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Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation. (C) 2003 Elsevier Inc. All rights reserved.
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