Journal
JOURNAL OF VIROLOGY
Volume 78, Issue 14, Pages 7818-7822Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.78.14.7818-7822.2004
Keywords
-
Categories
Funding
- NIAID NIH HHS [R01-AI51463, R01 AI051463] Funding Source: Medline
Ask authors/readers for more resources
We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available