Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 21, Issue 7, Pages 678-692Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/02652030410001711304
Keywords
mycotoxins; Fusarium; deoxynivalenol; fumonisin B1; zearalenone; maize; accelerated solvent extraction (ASE); solid-phase extraction; atmospheric pressure chemical ionization tandem mass spectrometry (APCI-MS/MS)
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A method for the simultaneous quantitative determination of deoxynivalenol ( DON), fumonisin B1 (FB1) and zearalenone (ZEN) in maize by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCIMS/MS), using stable isotopically labelled and structural analogues internal standards, is described. The procedure involves accelerated solvent extraction followed by two solid-phase clean-up steps on strong anion exchange resin and a Mycosep(R) column. Typical recoveries were calculated by spiking blank maize at three different concentrations for deoxynivalenol (200, 400 and 1000 mug kg(-1)) at 70%, for fumonisin B1 (100, 200 and 1000 mug kg(-1)) at 90%, and for zearalenone (50, 100 and 200 mug kg(-1)) at 40%. LC-APCIMS/MS analyses were realized in collision-induced dissociation on an ion-trap instrument to provide a high degree of selectivity and sensitivity. Extraction of ions from two transition reactions, monitored by LC-APCIMS/MS for each analyte, enabled a limit of detection for DON, FB1 and ZEN at, respectively, 10, 20 and 3 mug kg(-1), and a limit of quantification at, respectively, 50, 50 and 10 mug kg(-1). The robustness of the method was also evaluated with the analysis of wheat samples.
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